中三 生物試卷 (F3 Biology Past Paper)

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A Multiple choice questions (7 marks)
Which of the following is/are catabolic reaction(s)?
(1) amino acids + water polypeptide
(2) triglyceride → fatty acids + glycerol
(3) glucose + oxygen → carbon dioxide + water + energy
A. (1) only B. (2) only C. (1) and (2) only
Which of the following is not a property of enzymes?
A. Enzymes are not consumed in the reaction.
B. A small amount of enzymes is enough to catalyse a reaction.
Enzymes increase the activation energy of the reaction.
Enzymes can only work on one specific type of substrates.
total volume of oxygen
(arbitrary unit)
total volume of oxygen
(arbitrary unit)
1 cm³ of 0.3% amylase solution is added to 1 cm³ of 1% starch solution. After 20 minutes, the mixture
is tested with iodine test, Benedict's test and a protein test strip. What would be the possible result?
Iodine test Benedict's test Protein test strip
A student carried out an experiment to study the effect of temperature on the activity of catalase. A cube
of pig liver tissue with sides of 0.5 cm was put into 5 cm³ of hydrogen peroxide solutions at different
temperatures. He collected the oxygen produced and recorded the total volume of oxygen produced
regularly. Which of the following graphs shows the most likely results of the experiment?
produced (arbitrary unit)
total volume of oxygen
total volume of oxygen
produced (arbitrary unit)
D. (2) and (3) only
The graph below shows the effect of pH on the activities of three enzymes.
enzyme 2 enzyme 3
A. enzyme P
C. cnzyme R
enzyme activity
Which of the following statements about enzymes 1, 2 and 3 is incorrect?
A. Enzyme 1 is more active than enzyme 2 below pH 5.
B. Enzymes 2 and 3 are denatured at pH 12.
Enzymes 1, 2 and 3 can work at pH 7.
D. Enzymes 1, 2 and 3 have different optimum pH.
(1) and (2) only
(1) and (3) only
(2) and (3) only
D (1), (2) and (3)
The table below shows four choices of enzymes to be included in biological washing powder, and their
optimal conditions.
Which enzyme is the most suitable to be used in the washing powder?
D. enzyme S
Optimal temperature (°C)
Which of the following statements about the factors affecting enzyme activity are incorrect?
(1) Low temperatures decrease enzyme activity because enzymes are denatured.
(2) High temperatures decrease enzyme activity because the enzyme and substrate molecules cannot
collide with one another.
(3) Unsuitable pH decreases enzyme activity because the activation energy of the reaction becomes
(a) A glowing splint was put near the mouth of the test
tubes after some time. State the expected results in
tubes A and B.
Conventional questions (11 marks)
Many animal tissues contain the enzyme catalase, which catalyses the conversion of hydrogen peroxide
to oxygen and water. Mary carried out an experiment to study the action of catalase in liver tissue. The
diagram below shows the set-ups of her experiment.
(b) What can Mary conclude from the results in (a)?
2. The diagram below shows the energy profile for an enzymatic reaction.
energy level
boiled liver
progress of reaction
hydrogen peroxide
(a) Is the reaction a catabolic reaction or an anabolic reaction? Explain your answer.
fresh liver
(b) Sketch a curve on the diagram to show the changes in energy level when the enzyme is absent.
3. Biological washing powders contain proteases. Janet carried out an investigation to compare the
protease activities in three brands (A, B, C) of biological washing powders. She added solutions of
different washing powders into the wells of a milk agar plate as shown in the diagram below. Proteases
help digest milk proteins to colourless products.
(b) State one controlled variable in Janet's investigation.
(a) What should Janet measure so as to compare the protease activities in the washing powders?
(d) Name another commercial product that contains proteases.
(c) What should Janet add into the well which served as a control? What was the purpose of setting up a
control in this investigation?
Multiple-choice questions
Conventional questions
A: The glowing splint does not relight.
B: The glowing splint relights.
Catalase in the liver tissue is destroyed / denatured by boiling.
(or other reasonable answers)
energy level
It is an anabolic reaction
because the energy level of the products is higher than that of the reactants, showing that energy is
absorbed during the reaction.
progress of reaction
Curve having a higher peak than the original curve
Energy levels of reactants and products remaining the samc
without enzyme
with enzyme
The diameter of the clear zones formed around the wells after a period of time
Concentration of washing powder solutions (or other correct answers)
Distilled water
To show that the formation of clear zones was caused by substances in the washing powders.
Contact lens cleaners / Meat tenderizers (or other correct answers)